We would like to show you a description here but the site won’t allow us. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. 10. 3: The cuvette chamber. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. TRF as Secondary Detection Mode: TRF is supported as a secondary detection mode by these readers: Gemini XS, EM, XPS, M2, & FlexStation. Gemini XPS Fluorescence Microplate Reader . BMG LABTECH. 40266 Email: drugdiscoverytech@lifetech. Then the working solution (50μL) was added to the plate for 30 min treatment without light at room temperature and then the stop solution (50μL) was added. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. This can increase throughput and efficiency, thus saving time and space in the lab. Incubation. The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. . The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. In the presence of ACE, the PL intensity was increasing by time, while the PL intensity sustained a low and stable fluorescence in 3 hours in the absence of ACE. Type Clear: Add to Cart. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Connecting Instrument Cables The power cord and USB cable connect to the ports on the rear of the instrument. 1. 58% for the detection module and 2. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Align the two slots on the bottle holder with the two pegs on the rail. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. 00 USD. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The SpectraMax microplate readers support a wide variety of assay categories including . 3 foot) See. The instrument will read the plate, then the relative fluorescence units (RFUs) will be displayed in the Plate section of SoftMax Pro Software. The SpectraMax 190 reader can be integrated with Molecular . The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. Features. The emitted• Fluorescence Intensity (FI) • Luminescence (Lum) • Time-Resolved Fluorescence (TRF) • Fluorescence Polarization (FP) The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. 1 fmol/well 384-well plate) Luminescence Specifications Wavelength Range 300 to 700 nm Dynamic Range < 6 decades Sensitivity 10 amol ATP typical (flash) Fluorescence Polarization Specifications Light Source. for the plate. Plate reader. free tracer decreases, resulting in an overall increase in RFU values. 25 to 3. Step 5: Click the software’s Read button. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing. Conclusions. The SpectraMax iD3 reader detects fluorescence using monochromators only. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western. Important things to consider when purchasing a multimode microplate reader. 25 to 3. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. They are widely used in research, drug discovery, [1] bioassay validation, quality control and manufacturing processes in the pharmaceutical. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. The SpectraMax i3x reader provides theThis Molecular Devices SpectraMax iD5 Microplate Reader is made in Oct. 0 out of 5. ~ Bio-Tek ELx808 Microplate Reader $2,795. Luminescence was read on the SpectraMax i3x reader and the SpectraMax L reader. Figure 2. This plate reader is capable of monitoring UV and visible absorbencies, fluorescence, and luminescence. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. 5°C at 37°C. Microplate Readers. Package performance tests. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Wavelength optimization. luminescence, and fluorescence microplate readers, and the SpectraMax® Paradigm® Multi-Mode Detection Platform. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Plate Shaking Linear, orbital, double-orbital Dimensions (H x W x D) 12 x 15 x 15 in. The method is based on. Tecan Infinite F200 Fluorescence Microplate Reader. A microplate reader is a device used to detect chemical, biological, or physical reactions by measuring emitted light. Featuring temperature control up toThe SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. SpectraMax ® M2/M2e: Not Capable: Validated:. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. The chamber contains. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. The Spectr. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Tristar 3 Multimode Microplate Reader. Multimode microplate readers offer flexibility, and ease of use. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. Wide range of applications The VersaMax reader covers a wide range of applications:Molecular Devices SpectraMax M2 Multimode Plate Reader. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Luminex Magpix. Data from The SpectraMax® iD3 Multi Mode Microplate . I am using the filter pair of 480/535 and trying to measure fluorescence. An assay plate containing a 15-point standard curve was read on the SpectraMax. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. 53 - 60 kg, (117-132 lb), depending on configuration. Molecular Devices Spectra Max Gemini XS fluorescence Microplate reader. The assay is carried out in a standard 96-well plate. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. The IC50s by cell rounding and LysoTracker assays were 0. of each sample medium was transferred to a 96-well flat-bottom plate. . Peeking into a research lab today, it would be rare to see a line-up of microplate readers on a bench, each capable of performing only a single type of measurement. Transcreener ® ADP. Figure 6: Transcreener ADP2 FI assay principle. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. 67. Fluorescent Polarization: Solid black plates, as above. The SpectraMax® ABS Plus Microplate Reader can run . The SoftMax Pro Software validation package and IQ/OQ validation protocols include tools for FDA 21 CFR Part 11 compliance. Spectramax Multi-Mode Microplate Reader Performance Specifications. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreThe SpectraMax iD3 reader measures absorbance, fluorescence, and luminescence. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. The FI assay was performed using the SpectraMax ® M2 Multi-Mode Microplate Reader. Description The onboard microprocessor calculates and reports the absorbance, % transmittance, and/or fluorescence for each well of a microplate or from the cuvette port. Also refer to this Multimode Reader Comparison Chart. BMG Labtech - THERMOstar. D 59 A. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader andMicroplate Readers. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). The Biophysical Resource at Keck Facility offers the fluorescence plate reader as an "open access" instrument that can be used by trained users. Model Description. FL1 Fluorescence Plates, and LM1 Luminescence Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Microplate readers’ standard detection modes. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or spectrophotometer. Molecular Devices SpectraMax™ M2e Microplate Reader w/ Warranty. It features a modular design that allows users to add or change detection cartridges according to their needs. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Select the lab user profile. 1. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. We would like to show you a description here but the site won’t allow us. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Factors that contribute to an instrument’s sensitivity, or limit of detection, include background noise from the detector and electronics, the type of detector that is used, and the instrument’s configuration and. Download Application Note Plate reader settings for NanoOrange assay. SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use: $20/hr:Reads a 96-well plate in 15 s, a 384-well plate in. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应 Instrument settings used with the SpectraMax M5 Microplate Reader. The microplate reader will read the plate and the relative fluorescence units will be displayed in the Plate section. 2 only) SpectraMax M-series Microplate readers. 7. Assay plates containing the 15-point standard curve were read on the Molecular Devices' SpectraMaxš M2 Microplate Reader. Microplate Readers. 67. Driver installation. Linear, Orbital Slow, and Orbital Fast. Biotek FLx800 Fluorescence Microplate Reader. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The plate reader offers a fast, simple readout of average fluorescence per well, while the cytometer gives more detailed information, including total cell number and. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading. The plate reader. Because. Therefore, ADP production is proportional to an increase in fluorescence. 00. $ 9,499. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. Typical settings for SpectraMax readers with fluorescence detection mode are shown. 2. Type: Microplate Reader. 2018. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. g. Fixes: General:SpectraMax M2 and M2e Multi-Mode. Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideReader Documentation Version No. NanoQuant plate. STAKMAX. We use Spectramax M5 microplate reader in our lab. Plate chamber design of the SpectraMax category 1 microplate readers. (in addition to fluorescence imaging) on the SpectraMax i3x Multi-Mode Microplate Reader. FI assay. We therefore gathered salient. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Fluorescence is measured at time zero and again after one-hour of incubation at 37°C in a fluorescence microplate reader. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. Offer Ends In: 40 Days 05: 02: 03. This enables the standard curve to be plotted and sample concentrations to be calculated automatically by SoftMax. The SPECTRAmax® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics that permit experimental determinations at low-level detection limits. This reader's dual monochromators for. 0 out of 5 $ 14,499 $ 13,995-13%. E Models. #4513: Plate Reader: A multi-detection microplate reader configured to measure the output of the UDP tracer is required. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. WinKQCL ® Software can be used with a compatible microplate reader to run the template and. 5. Customer Satisfaction is. M2. With optimized reagents, option to operate the. SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). SpectraMax ® M2 Multi-Mode Microplate Reader. #6590-0108) Fluorescence detection using the SpectraMax iD5 reader’s monochromators for wavelength selection was compared to that using a filter pair specific for the Alexa594 tracer. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and. Plate reader settings for NanoOrange assay. SpectraMax M2 and use thereof is covered by issued (U. 0) OD (0. Deviations in boronic acid concentration were necessary when the K ARS for a boronic acid was too low (<100 M. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. A 96-well plate scheme was selected for the measurement mode and the wells selected for targeting the respective colonies arrayed. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. It is also extremely flexible and can measure samples held in the 96, 384, 1536 and other. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. The SpectraMax Paradigm Detection Cartridge design utilizes an energy intensity optimization technique that allows the user to read up to seven logs of sample concentration in a single pass of a plate without any manual intervention. It also. The purple holder is used for reading non-transparent plates from the top (e. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. 0 out of 5 $ 3,895. 04 to 3. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Figure 2. 47 and 0. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. Assay plates containing the 15-point standard curve were read on the Molecular Devices' SpectraMaxš M2 Microplate Reader. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. The optical systems use two scanning monochromators soUnlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Insert the snorkel into the bottle and then insert the snorkel into the snorkel clamp. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. The superior optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) that reduces background noise for excellent sensitivity and a wide dynamic range. Update Driver. Multimode Reader Comparison Chart. 6 % of reading 4. 1. SpectraMax Microplate Readers. White plates reflect the light, enabling even low levels of Luminescence to be detected. 2. It is typically used to determine the concentration of nucleic acids and proteins. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Third this recommendation. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). For example, microplates containing different samp les can be read using the same orThe SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. SpectraMax M2 plate reader (Molecular Devices) was employed to measure the absorbance at 490 nm and 680 nm. 4. SpectraMax Microplate Readers. SpectraMax Spectrofluorometers. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . With exceptional signal-to-noise ratio and a lower detection limit. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. We suggest Corning 384-well plates Cat. The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. Settings for SpectraMax iD3 Multi-Mode Microplate Reader. 304 signal level saturation. Molecular Devices SpectraMax M2 ; Molecular Devices. D. Fluorescence measurements no longer require you to adjust the gain. SpectraMax Glo Steady-Luc Reporter Assay Kit. olecular saka. It can measure absorbance from 230 -1000 nm. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax ® Pro. UsethecuvetteportforAbsorbance,Fluorescence,andLuminescence(secondary mode)readmodes. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 0 Concentration Dynamic Range 105 RFU MT oltage ignal Adjustable PMT gain High. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. The EarlyTox Live/Dead Assay Kit contains two markers for live or dead cells that are suitable for use with mammalian cells. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. Double-click the unknown device with the yellow warning icon. In the lower half of the window, left side, there is a box labeled reader. D 4/21/06 9:53:29 AM . A solution for low volume applications. 303 unable to cal dark current. In the M2, M5 and Plus 384, the light. Two important settings to obtain the best results in TRF assays. SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Additionally, all BMG LABTECH microplate readers offer the possibility to measure in very small volumes, down to 2 µl of the sample by using the LVis Plate. SpectraMax M2. Microplate readers’ standard detection modes are. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). 020 OD 2. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. The SpectraMax iD3 reader measures absorbance, fluorescence, and luminescence. The SpectraMax i3 Multi-Mode Plate Reader enables characterization of proteins by measuring intrinsic tryptophan fluorescence. SpectraTest FL1 Fluorescence Validation . patent nos. Suggested plates for best data: Fluorescence-top read; Solid Black flat-bottom for Excitations above 350 nm;. A whole well fluorescence imaging system that can collect information from all cells inside the well, from 6- to 384-well formats. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Listing: #4772697. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. 10. It may be named "Pothos". 8 Customer Reviews. A solution for low volume applications. Perform the scan and note the wavelength that gives maximum emission. SPECTRAmax GEMINI-XS spectrofluorometer was able to detect a specific GFP signal at all cell numbers as determined by One Way Analysis of Variance. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. Additionally, by issuing a single read command, combined absorbance and fluorescence intensity assays can be run. The SpectraMax® M2 and M2e Multimode. 67. Molecular Devices SpectraMax M2 Multimode Plate Reader. (a) Chemical structures of 1 (HADP) and its analogues 2–4. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Absorbance Fluorescence and fluorescence polarization Luminescence. 2. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence microplate readers are as Fluorescence Validation Test Plates provide validation of the system’s optical characteristics. Reads various kinds of 96-wells plates, and is equipped with shaking function. ~ Bio-Tek ELx808 Microplate Reader $2,795. Absorbance cuvette port. The plate is set up for reading and images are acquired according to specified parameters. e. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. g. 0 × 107) RLU 0. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. orporation. Three data types for FP read types are also being introduced with v4. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. This opens the Settings window. The OliGreen assay from Molecular Probes, when run on a Gemini XS or Gemini EM fluorescence microplate reader with SoftMax Pro software, is a quick, sensitive detection method for single-stranded DNA. 0 software was used to handle data analysis and control each instrument. 119. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. on SpectraMax M2 fluorescence plate reader (Molecular Devices, CA, USA): excitation wavelength 320nm, emission wavelength 470nm. Using standard fluorescence optimization can take hours or days to determine the best wavelengths to use for excitation. Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. Evaporation protection (humidity cassette) Te-Cool™ (active temperature regulation 18-42 °C) Integrated lid handling. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. spr * SpectraMax i3(x) SpectraTest ABS2 v1. 0 × 103 to 2. Microplate Reader Shelf. 0) OD (500 to 1100) RFU (5. Self Service Available. 8: theMicroplate Reader & Luminometer Selection Guide. (b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. Absorbance (monochromator). The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. SpectraMax readers are equipped with PathCheck Technology, which measures the pathlength in each well of a microplate and normalizes the absorbance. . S. The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. Appendix A. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. The percent The percent (4) Asiaticoside reverses M2 phenotype macrophage polarization-evoked osteosarcoma cell malignant behaviour by TRAF6/NF-κB inhibition Pharmaceutical Biology December 31, 2022 Dang-ke. dual monochRomatoRs foR assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. item 1 Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader - JVSHJH O Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader - JVSHJH O $4,999. 0 out of 5 $ 2,999-20%. 0. The flexibility of a high end multimode plate reader with environmental control. The plate blank’s RFU values should be subtracted from the RFUs of all samples of the plate. Figure 1. Perform the following steps to install the drivers: Start > Control Panel > System. on SpectraMax M2 fluorescence plate reader (Molecular Devices, CA, USA): excitation wavelength 320nm, emission wavelength 470nm. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). The SpectraTest fluorescence test plate (FL-1) can be used by Gemini XPS & M2 readers. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. Note: In this user guide, SpectraMax ® refers to several SpectraMax . As new assays are developed or yourFor TRF assays, you will need a fluorescence plate reader equipped with a time-resolved fluorometry option and a 330 nm excitation filter and emission filters capable of detecting 647 nm (620/40) and 665 nm (665/ 7. 306 plate air cal fail,low light. Microplate Readers Molecular Devices SpectraMax M2 Multimode Plate Reader. SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence and luminescence. . fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). The GloMax® line includes high-performance plate readers with a range of detection options.